abstract：:In chronic eosinophilic leukemia, the transforming oncoprotein FIP1L1-PDGFRA is a major target of therapy. In most patients, the tyrosine kinase inhibitor (TKI) imatinib induces complete remission. For patients who are intolerant or resistant, novel TKIs have been proposed. We examined the in vitro effects of 14 kinas...

journal_title：Experimental hematology

authors： Sadovnik I,Lierman E,Peter B,Herrmann H,Suppan V,Stefanzl G,Haas O,Lion T,Pickl W,Cools J,Vandenberghe P,Valent P

更新日期：2014-04-01 00:00:00

abstract：:It has been shown that in xenotransplantation of human cells into immunodeficient mice, the mouse strain background is critical. For example, the nonobese diabetic (NOD) strain is most efficient, the BALB/c is moderate, and the C57BL/6 is inefficient for human cell engraftment. We have shown that the NOD-specific poly...

journal_title：Experimental hematology

authors： Iwamoto C,Takenaka K,Urata S,Yamauchi T,Shima T,Kuriyama T,Daitoku S,Saito Y,Miyamoto T,Iwasaki H,Kitabayashi I,Itoh K,Kishimoto J,Kohda D,Matozaki T,Akashi K

更新日期：2014-03-01 00:00:00

abstract：:The tumor suppressor p53 is thought to play a role in megakaryocyte (MK) development. To assess the influence of the p53 regulatory pathway further, we studied the effect of RG7112, a small molecule MDM2 antagonist that activates p53 by preventing its interaction with MDM2, on normal megakaryocytopoiesis and platelet ...

journal_title：Experimental hematology

authors： Iancu-Rubin C,Mosoyan G,Glenn K,Gordon RE,Nichols GL,Hoffman R

更新日期：2014-02-01 00:00:00

abstract：:HIV-derived lentiviral vectors have been used widely to transduce non-dividing cells, such as hematopoietic stem cells (HSCs), in the setting of gene therapy. In this study, we screened lentiviral vectors for their ability to drive expression of the murine MHC class II chaperone, invariant chain (Ii) and a GFP reporte...

journal_title：Experimental hematology

authors： Wang N,Rajasekaran N,Hou T,Lisowski L,Mellins ED

更新日期：2013-11-01 00:00:00

abstract：:Infusion of large numbers of donor regulatory T cells (Tregs) is an effective approach to suppress graft-versus-host disease (GvHD). We have reported previously that enhancing the killing activity of CD25(+) Tregs by decoration with short-lived Fas-ligand (FasL) protein (killer Tregs) is effective in abrogation of aut...

journal_title：Experimental hematology

authors： Yolcu ES,Kaminitz A,Mizrahi K,Ash S,Yaniv I,Stein J,Shirwan H,Askenasy N

更新日期：2013-10-01 00:00:00

abstract：:Neutropenia is common to both Hermansky-Pudlak syndrome type 2 and canine cyclic hematopoiesis (CH) which are caused by mutations in the AP3B1 gene. The purpose of this study was to determine if pearl mice were neutropenic. Complete blood counts (CBCs) and bone marrow differential counts, colony forming unit (CFU) ass...

journal_title：Experimental hematology

authors： Vallejo MO,Niemeyer GP,Vaglenov A,Hock T,Urie B,Christopherson P,Lothrop CD Jr

更新日期：2013-10-01 00:00:00

abstract：:Patients with cytopenias and a cellular bone marrow can be a diagnostic and therapeutic challenge. Previous reports suggested a role for progenitor assays for diagnosis and predicting response to therapy. We report the results of Burst-forming unit-erythroid (BFU-E) assays in 48 consultative cases of single or multili...

journal_title：Experimental hematology

authors： DeZern AE,Pu J,McDevitt MA,Jones RJ,Brodsky RA

更新日期：2013-09-01 00:00:00

abstract：:Hematopoietic stem cell transplantation (HSCT) is an established treatment for multiple myeloma (MM), a plasma cell malignancy. To identify an improved pretransplant conditioning regimen, we investigated the cytotoxicity of gemcitabine (Gem) and clofarabine (Clo) combinations toward MM cell lines and patient cell samp...

journal_title：Experimental hematology

authors： Valdez BC,Wang G,Murray D,Nieto Y,Li Y,Shah J,Turturro F,Wang M,Weber DM,Champlin RE,Qazilbash MH,Andersson BS

更新日期：2013-08-01 00:00:00

abstract：:Nasal natural killer-T cell lymphoma (NNKTL) is associated with the Epstein-Barr virus and has distinct histologic features, such as angiocentric and polymorphous lymphoreticular infiltrates that contain too many cell types, including tumor and inflammatory cells. We have shown previously that intercellular adhesion m...

journal_title：Experimental hematology

authors： Takahara M,Nagato T,Komabayashi Y,Yoshino K,Ueda S,Kishibe K,Harabuchi Y

更新日期：2013-08-01 00:00:00

abstract：:Sustained NF-κB activation is often observed in acute myeloid leukemia (AML); therefore, proteasome inhibition has been proposed to efficiently target AML cells. In this study, we questioned whether leukemic stem cell-enriched CD34(+) cells are sensitive to the proteasome inhibitor bortezomib. Surprisingly, we observe...

journal_title：Experimental hematology

authors： Bosman MC,Schuringa JJ,Quax WJ,Vellenga E

更新日期：2013-06-01 00:00:00

abstract：:Autotaxin (ATX) has been reported to act as a motility and growth factor in a variety of cancer cells. The ATX protein acts as a secreted lysophospholipase D by converting lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA), which signals via G-protein-coupled receptors and has important functions in cell mig...

journal_title：Experimental hematology

authors： Ortlepp C,Steudel C,Heiderich C,Koch S,Jacobi A,Ryser M,Brenner S,Bornhäuser M,Brors B,Hofmann WK,Ehninger G,Thiede C

更新日期：2013-05-01 00:00:00

abstract：:A modified regimen of high-dose cyclophosphamide (CTX) plus cyclosporine (CsA) was adopted for patients with severe or very severe aplastic anemia, and the effectiveness was compared with a regimen of antithymocyte globulin (ATG) plus CsA. A total of 121 patients enrolled in this study received either CTX plus CsA (CT...

journal_title：Experimental hematology

authors： Zhang F,Zhang L,Jing L,Zhou K,Wang H,Peng G,Li Y,Li Y,Li J,Ye L,Shi L,Fan H,Zhao X,Chu Y,Hao Y,Wang J

更新日期：2013-04-01 00:00:00

abstract：:Interferon (INF)-α was the maintenance treatment of choice after autologous stem cell transplantation in multiple myeloma in the past, but currently Thalidomide is commonly used. In this prospective study, the implications of the various types of maintenance therapy on the patients T cell pattern and activation status...

journal_title：Experimental hematology

authors： Herth I,Witzens-Harig M,Beckhove P,Hose D,Meissner T,Neuber B,Engelhardt M,Haas J,Neben K,Ho AD,Klein B,Goldschmidt H,Hundemer M

更新日期：2013-03-01 00:00:00

abstract：:It has been demonstrated that genomic alterations of cells in the hematopoietic microenvironment could induce myelodysplastic syndromes (MDS) with ineffective hematopoiesis and dysmorphic hematopoietic cells, and subsequent transformation to acute myeloid leukemia. This investigation is the first attempt to correlate ...

journal_title：Experimental hematology

authors： Oliveira FM,Lucena-Araujo AR,Favarin Mdo C,Palma PV,Rego EM,Falcão RP,Covas DT,Fontes AM

更新日期：2013-02-01 00:00:00

abstract：:Retroviral gene marking has been used successfully in preclinical and clinical transplantation settings. Highly sensitive techniques for vector insertion-site determination, such as linear amplification-mediated polymerase chain reaction (LAM-PCR) in conjunction with next-generation sequencing, have been introduced to...

journal_title：Experimental hematology

authors： Cornils K,Bartholomae CC,Thielecke L,Lange C,Arens A,Glauche I,Mock U,Riecken K,Gerdes S,von Kalle C,Schmidt M,Roeder I,Fehse B

更新日期：2013-01-01 00:00:00

abstract：:The concept that mesenchymal stromal cells (MSCs), a component of the hematopoietic microenvironment, can be a target for alloreactive effector cells in the context of graft-vs-host disease has not been investigated in detail. Mixed lymphocyte reaction (MLR) supernatant was used to mimic the inflammatory milieu induce...

journal_title：Experimental hematology

authors： Fasslrinner F,Wobus M,Duryagina R,Müller K,Stopp S,Wehner R,Rauner M,Hofbauer LC,Schmitz M,Bornhäuser M

更新日期：2012-11-01 00:00:00

abstract：:Increasing evidence points to endoglin (Eng), an accessory receptor for the transforming growth factor-β superfamily commonly associated with the endothelial lineage, as an important regulator of the hematopoietic lineage. We have shown that lack of Eng results in reduced numbers of primitive erythroid colonies as wel...

journal_title：Experimental hematology

authors： Baik J,Borges L,Magli A,Thatava T,Perlingeiro RC

更新日期：2012-10-01 00:00:00

abstract：:Myelodysplastic syndrome is a clonal process characterized by ineffective hematopoiesis and progression to acute leukemia. Although many myelodysplastic syndrome and leukemic patients have compromised immunity, the role of underlying mutations in regulating immune function is poorly understood. Recent studies show tha...

journal_title：Experimental hematology

authors： Puthiyaveetil AG,Heid B,Reilly CM,HogenEsch H,Caudell DL

更新日期：2012-08-01 00:00:00

abstract：:Myelodysplastic syndromes (MDS) are clonal stem cell diseases of the bone marrow characterized by abnormalities in maturation of hematopoietic cells of all lineages. MDS patients frequently have lower lipids and high rates of apoptosis and p53 (TP53) expression. An association between the reduced lipids in MDS and the...

journal_title：Experimental hematology

authors： Ellis MH,Baraf L,Shaish A,Har-Zahav A,Harats D,Ashur-Fabian O

更新日期：2012-07-01 00:00:00

abstract：:Chronic lymphocytic leukemia (CLL) is a heterogeneous disease characterized by defects in the DNA damage response and apoptosis. Among the factors involved in these pathways, we focused on the enzyme poly(ADP-ribose) polymerase 1 (PARP1) and on its substrate Che-1 by evaluating their basal expression and functional ch...

journal_title：Experimental hematology

authors： Bacalini MG,Tavolaro S,Peragine N,Marinelli M,Santangelo S,Del Giudice I,Mauro FR,Di Maio V,Ricciardi MR,Caiafa P,Chiaretti S,Foà R,Guarini A,Reale A

更新日期：2012-03-01 00:00:00

abstract：:Disruption of ribosomal proteins is associated with hematopoietic phenotypes in cell culture and animal models. Mutations in ribosomal proteins are seen in patients with Diamond Blackfan anemia, a rare congenital disease characterized by red cell aplasia and distinctive craniofacial anomalies. A zebrafish screen uncov...

journal_title：Experimental hematology

authors： Taylor AM,Humphries JM,White RM,Murphey RD,Burns CE,Zon LI

更新日期：2012-03-01 00:00:00

abstract：OBJECTIVE:Thrombopoietin stimulates megakaryopoiesis and platelet production by binding to its receptor, Mpl, on hematopoietic progenitor cells. Previously, a murine VB22B minibody for Mpl was shown to stimulate megakaryocyte colony formation in vitro and increase the platelet count in cynomolgus monkeys. In this study...

journal_title：Experimental hematology

authors： Matsuki E,Miyakawa Y,Yamane A,Okamoto S

更新日期：2011-08-01 00:00:00

abstract：OBJECTIVE:Our laboratory reported that the angiotensin converting enzyme inhibitor captopril improves erythroid recovery from total body irradiation (TBI) in mice when administered after irradiation. However, captopril administered before TBI attenuates erythroid recovery. Here we investigate captopril and radiation re...

journal_title：Experimental hematology

authors： Barshishat-Kupper M,Mungunsukh O,Tipton AJ,McCart EA,Panganiban RA,Davis TA,Landauer MR,Day RM

更新日期：2011-03-01 00:00:00

abstract：OBJECTIVE:Polo-like kinase 1 (Plk1) is a regulator of the cell cycle that has been implicated in the pathology of many cancers. We have investigated whether this kinase plays a role in multiple myeloma (MM) using the Plk1 inhibitor BI 2536. MATERIALS AND METHODS:We have used six MM cell lines and six patient-derived s...

journal_title：Experimental hematology

authors： Stewart HJ,Kishikova L,Powell FL,Wheatley SP,Chevassut TJ

更新日期：2011-03-01 00:00:00

abstract：OBJECTIVE:Cyclosporine A (CsA), known as an effective immunosuppressive agent, is widely used in clinical fields. Mesenchymal stem cells may exert immunomodulatory effects on the immune system, but the exact mechanisms underlying them remain controversial. Here we investigated whether human adipose tissue-derived mesen...

journal_title：Experimental hematology

authors： Shi D,Liao L,Zhang B,Liu R,Dou X,Li J,Zhu X,Yu L,Chen D,Zhao RC

更新日期：2011-02-01 00:00:00

abstract：OBJECTIVE:These studies were performed to test the hypothesis that DNMT1 is required for maintenance of DNA methylation and repression of the γ-globin gene in adult-stage erythroid cells. MATERIALS AND METHODS:DNMT1 levels were reduced by nucleofection of small interfering RNA targeting DNMT1 in chemical inducer of di...

journal_title：Experimental hematology

authors： Banzon V,Ibanez V,Vaitkus K,Ruiz MA,Peterson K,DeSimone J,Lavelle D

更新日期：2011-01-01 00:00:00

abstract：OBJECTIVE:Morbidity and mortality occurring in patients with multiple myeloma, AL amyloidosis, and light chain deposition disease can result from the pathologic deposition of monoclonal immunoglobulin light chains (LCs) in kidneys and other organs. To reduce synthesis of such components, therapy for these disorders typ...

journal_title：Experimental hematology

authors： Phipps JE,Kestler DP,Foster JS,Kennel SJ,Donnell R,Weiss DT,Solomon A,Wall JS

更新日期：2010-11-01 00:00:00

abstract：OBJECTIVE:Several transcription factors determine the cell fate decision between granulocytes and monocytes, but the upstream signal transduction pathways that govern myelopoiesis are largely unknown. Based on our observation of aberrant myeloid cell representation in hematopoietic tissues of 12/15-lipoxygenase (12/15-...

journal_title：Experimental hematology

authors： Kinder M,Thompson JE,Wei C,Shelat SG,Blair IA,Carroll M,Puré E

更新日期：2010-11-01 00:00:00

abstract：OBJECTIVE:Myeloid ectropic viral integration site 1 (MEIS1) is a Hox cofactor known for its role in development and is strongly linked to normal and leukemic hematopoiesis. Although previous studies have focused on identifying protein partners of MEIS1 and its transcriptionally regulated targets, little is known about ...

journal_title：Experimental hematology

authors： Xiang P,Lo C,Argiropoulos B,Lai CB,Rouhi A,Imren S,Jiang X,Mager D,Humphries RK

更新日期：2010-09-01 00:00:00

abstract：OBJECTIVE:Differentiation-inducing therapy by agents such as 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) represents a useful approach for the treatment of acute myelogenous leukemia (AML). We previously showed that Gemini-23-yne-26,27-hexafluoro-D(3) inhibited the proliferation of MCF-7 breast cancer cells in associat...

journal_title：Experimental hematology

authors： Yang J,Ikezoe T,Nishioka C,Ni L,Koeffler HP,Yokoyama A

更新日期：2010-08-01 00:00:00

abstract：:Circulating CD14(+) monocytes are originated from hematopoietic stem cells in the bone marrow and believed to be committed precursors for phagocytes, such as macrophages. Recently, we have reported a primitive cell population termed monocyte-derived multipotential cells (MOMCs), which has a fibroblast-like morphology ...

journal_title：Experimental hematology

authors： Seta N,Kuwana M

更新日期：2010-07-01 00:00:00

abstract：:In slightly more than a decade of stem cell plasticity research, 24 peer-reviewed articles have demonstrated plasticity across organ and/or embryonic lineage boundaries at the single-cell level, with only 1 article showing negative results. These data, taken together with data about reversibility of gene restrictions ...

journal_title：Experimental hematology

authors： Theise ND

更新日期：2010-07-01 00:00:00

abstract：OBJECTIVE:CD34(+) cells, present within the bone marrow, have previously been shown to possess pancreatic endocrine potential. Based on this observation, we explored the capacity of CD34(+) cells derived in culture from the differentiation of human embryonic stem cells (hESC), for their in vivo pancreatic endocrine cap...

journal_title：Experimental hematology

authors： Goodrich AD,Ersek A,Varain NM,Groza D,Cenariu M,Thain DS,Almeida-Porada G,Porada CD,Zanjani ED

更新日期：2010-06-01 00:00:00

abstract：OBJECTIVES:To identify the regulatory sequences driving Gata1 expression in conventional dendritic cells (cDC). MATERIALS AND METHODS:The number and expression levels of Gata1, Gata1-target genes and hypersensitive site (HS) 2 (the eosinophil-specific enhancer)-driven green fluorescent protein (GFP) reporter of cDCs f...

journal_title：Experimental hematology

authors： Kozma GT,Martelli F,Verrucci M,Gutiérrez L,Migliaccio G,Sanchez M,Alfani E,Philipsen S,Migliaccio AR

更新日期：2010-06-01 00:00:00

abstract：OBJECTIVE:Multipotent germline stem (mGS) cells derived from neonatal mouse testis, similar to embryonic stem (ES) cells, differentiate into various types of somatic cells in vitro and produce teratomas after inoculation into mice. In the present work, we examined mGS cells for hematopoietic progenitor potential in vit...

journal_title：Experimental hematology

authors： Yoshimoto M,Heike T,Chang H,Kanatsu-Shinohara M,Baba S,Varnau JT,Shinohara T,Yoder MC,Nakahata T

更新日期：2009-12-01 00:00:00

abstract：OBJECTIVE:Mesenchymal stem cells (MSCs) are capable of modulating the immune system through interaction with a wide range of immune cells. This study investigates the hypothesis that interaction of MSCs with macrophages could play a significant role in their antiinflammatory/immune modulatory effects. MATERIALS AND ME...

journal_title：Experimental hematology

authors： Kim J,Hematti P

更新日期：2009-12-01 00:00:00

abstract：OBJECTIVE:Polycythemia vera (PV) is characterized by erythrocytosis associated with the presence of the activating JAK2(V617F) mutation in a variable proportion of hematopoietic cells. JAK2(V617F) is detected in other myeloproliferative neoplasms, does not appear to be the PV-initiating event, and its specific role in ...

journal_title：Experimental hematology

authors： Laubach JP,Fu P,Jiang X,Salter KH,Potti A,Arcasoy MO

更新日期：2009-12-01 00:00:00

abstract：OBJECTIVE:Common marmosets are considered experimental animals of primates useful for medical research. We developed several monoclonal antibodies (mAbs) directed to CD molecules to gain initial insight into the immune and hematopoietic systems of this organism, and analyzed the basic cellularity and characters of marm...

journal_title：Experimental hematology

authors： Kametani Y,Suzuki D,Kohu K,Satake M,Suemizu H,Sasaki E,Ito T,Tamaoki N,Mizushima T,Ozawa M,Tani K,Kito M,Arai H,Koyanagi A,Yagita H,Habu S

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVE:A complete process for mass generation of megakaryocytes from hematopoietic stem cells under serum-free conditions has great clinical potential for rapid platelet reconstruction in thrombocytopenia patients. We have previously reported on the generation of an optimized serum-free medium (serum-free hematopoie...

journal_title：Experimental hematology

authors： Chen TW,Hwang SM,Chu IM,Hsu SC,Hsieh TB,Yao CL

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVE:Our previous studies have demonstrated differentially expressed genetic signature patterns in adult peripheral blood (APB) monocytes (Mos) vs cord blood (CB) Mos. MATERIALS AND METHODS:In this study, we examined the differential gene expression profiles of APB vs CB immature dendritic cells (iDCs) and mature...

journal_title：Experimental hematology

authors： Jiang H,van de Ven C,Baxi L,Satwani P,Cairo MS

更新日期：2009-10-01 00:00:00